yabS [2019-08-27 13:05:43]

unknown

locus: BSU_00650

pI: 4.72

mw: 26.63 kDa

protein length: 245 aa

gene length: 738 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates: 73,106 → 73,843

The protein

Structure

• [PDB|3RAG] (from Alicyclobacillus acidocaldarius, 41% identity)

Expression and Regulation

Operons

• genes: [gene|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|spoIIE]-[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]-[gene|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT]-[gene|473F93EA5ADB78CD23C9C054B5D6B739078DDEEB|tilS]-[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]-[gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]
  description: [pubmed|22383849]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed early during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|16497325]

Biological materials

Mutant

• MGNA-B920 (yabS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1919 NBRP B. subtilis, Japan]
• BKE00650 (Δ[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATTCATTCCTCCTGGAG, downstream forward: _UP4_CGCTCAAGAAGGGGCATGCT
• BKK00650 (Δ[gene|C8AA53889FC8B389E1D921B762BD78990C7A8E57|yabS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATTCATTCCTCCTGGAG, downstream forward: _UP4_CGCTCAAGAAGGGGCATGCT

References

Research papers

• 22383849